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Oedema is considered a key pathogenic factor in the development of venous leg ulcers. The purpose of this study was to determine the localization of oedema in legs with ulcers. Twelve patients with 1...

Europe PMC is an archive of life sciences journal literature.

The fresh leaves and extract of the plant Chromolaena odorata are a traditional herbal treatment in developing countries for burns, soft tissue wounds and skin infections. We have previously shown that the extract had an effect on the growth and proliferation of keratinocytes and fibroblasts in culture. This study has demonstrated that Eupolin extract increased expression of several components of the adhesion complex and fibronectin by human keratinocytes. Using indirect immunofluorescence we found increased expression (dose-dependent) of laminin 5, laminin 1, collagen IV, and fibronectin. The expression of the b1 and b4 integrins was upregulated by the extract at low concentrations (0.1 and 1 µg/ml), but the expression was decreased at higher doses of Eupolin (10 µg-150 µg/ml).A number of clinical studies carried out by Vietnamese and international medical investigators have demonstrated the efficacy of this extract on the wound healing process. In this study we have shown that Eupolin stimulated the expression of many proteins of the adhesion complex and fibronectin by human keratinocytes. The adhesion complex proteins are essential to stabilise epithelium and this effect could contribute to the clinical efficacy of Eupolin in healing.

Fibroproliferative scars are characterized by abnormally high rates of collagen production, and increased levels of extracellular matrix (ECM) components as fibronectin and proteoglycans [1,2]. Quercetin, a dietary compound, has strong anti-oxidant and anti-tumor properties [3–7]. Previous studies have demonstrated that quercetin strongly inhibited keloid and hypertrophic scar-derived fibroblast proliferation, contraction and production of collagen [8]. We also demonstrated that quercetin suppressed both basal expression and activation of several key molecular proteins in the IGF-1 signaling pathway [9]. This study sought to investigate the inhibitory effects of quercetin on fibronectin production by keloid fibroblasts.

Abstract. Summary Background Linear IgA disease (LAD) is an IgA‐mediated subepidermal immunobullous disease of adults and children, with heterogeneous immu

Surgical researchers were among the first to describe the different phases of wound healing and the events in tissue repair and regeneration that were taking place during each phase. The understandin...

In this study, betamethasone disodium phosphate-loaded microparticles were fabricated for sustained release using poly(lactide-co-glycolide) (PLGA) by spray drying and emulsion solvent evaporation/...

Purpose. To investigate the in vitro and in vivo proliferative capacity of human conjunctival epithelial cells cultured in serum-free media, and to compare this with current methods that utilize se...

Smad signalling plays important roles in developmental and cancer biology as well as in fibropathogenesis. Its role in keloid biology is not known. Epithelial–mesenchymal interactions, originally des...

Clinicians have observed that keloids preferentially form in body areas subject to increased skin tension. We hypothesized a difference exists in the transcriptional response of keloid fibroblasts to...

Human dermal fibroblasts adhere well to a thermosensitive membrane surface at 37°C (figure, left) but detach at 25°C or below. The detached cells can be reattached for growth on a cell culture plate ...

Vascular endothelial growth factor (VEGF) plays an important role in angiogenesis during the wound healing process. As epithelial–mesenchymal interactions have been shown to regulate a plethora of ge...

Numerous experimental approaches in the field of dermatological research necessarily require the usage of human primary keratinocytes as immortal cell lines generally do not appropriately reflect the in vivo situation. However, the utilization of freshly isolated cells is often hampered by the limited accessibility of human skin biopsies and their derivation from donors of different age, gender and pathological background, as well as from different body sites. Recently it has been reported that the umbilical cord epithelium (UCE) expresses a cytokeratin pattern similar to human epidermis [1], although the general architecture of the umbilical cord epithelium significantly differs from the mammalian epidermis [2].

The use of human stem cells (SCs) is a promising novel approach for the treatment of many diseases and injuries. Umbilical cord and amniotic membrane represent ...

Summary. Background Keloids are pathological scars and, despite numerous available treatment modalities, continue to plague physicians and patients.Objecti

Please cite this paper as: Modulation of metallothionein isoforms is associated with collagen deposition in proliferating keloid fibroblasts in vitro. Experimental Dermatology 2010; 19: 987–993. Abs...

International Journal of Obesity - Cord lining progenitor cells: potential in vitro adipogenesis model

Summary. Background Keloids are fibroproliferative disorders characterized by increased deposition of extracellular matrix components. Stem cell factor (SC

Stem Cell Reviews and Reports - The stem cells involved in renewal of the corneal epithelium are located in the basal region of the limbus, a narrow transition zone surrounding the cornea. In many...

Stem Cell Reviews and Reports - Umbilical cord tissue is gaining attention as a novel source of multipotent stem cells because it is easily obtainable, ethically acceptable and the cells are...

Summary Background Keloid scarring is a dermal fibroproliferative disorder characterized by increased fibroblast proliferation and excessive production of collagen and extracellular matrix (ECM) com...

The primary hepatocyte is the best benchmark for drug biotransformation studies. However, due to the severe shortage of primary hepatocytes, there is a need for...

Europe PMC is an archive of life sciences journal literature.